Oocyte maturation is a critical component of in vitro embryo production. If not carried out in a precise manner under optimal conditions, subsequent fertilization and embryo development will be compromised. Here, we describe collection and in vitro maturation procedures in swine that maintain oocyte competence, resulting in successful embryo development following fertilization. These procedures can be used both for basic research purposes and large-scale production of mature oocytes for use in subsequent assisted reproductive technologies.
