A simple immobilization method forCandida rugosalipase on superparamagnetic Fe3 O4 nanoparticles is described. The Fe3 O4 nanoparticles were coated with PEI andCandida rugosalipase was adsorbed on these particles via electrostatic interactions. The immobilization resulted in marginal simultaneous purification. However, the immobilized preparation showed 110� higher transesterification activity in low-water media. It was also efficient in kinetic resolution of (�)-1-phenylethanol with eep of 99 % andE= 412 within 24 h.
