Gene therapy is a promising approach for the treatment of a variety of disorders including genetic diseases and cancer. Among the viral vectors used in gene therapy, the lentiviral vector, based on HIV-1, is the only integrative vector able to transduce nondividing cells. The first generation of lentiviral vector was �established in 1996. Since then, other generations of lentiviral vector packaging systems were developed to improve this first vector. In this chapter, we describe these different packaging systems, the generation of lentiviral vector from productive cells, the 293T cell line, and the transduction of myogenic cells with a lentiviral vector as well.
