Material/reagents:
• 2X binding buffer: 20 mM Tris pH 7.5 100 mM NaCl 2 mM EDTA 10% glycerol
• poly(dI-dC) (5 mg/ml in TE)
• BSA (10 mg/ml)
• 32P-labeled probe
• Buffer C: 20 mM HEPES pH 7.9 25% glycerol 420 mM NaCl 1.5 mM MgCl2
• 4% native polyacrylamide gel (50 ml): 5 ml 40% acrylamide (30:1) 2.5 ml 5X TBE 41.95 ml H20 0.5 ml 10% APS 50 ml TEMED
• 0.25X TBE running buffer
• Loading dye: 0.2% bromophenol blue 0.2% xylene cyanol 50% glycerol 0.5 mM DTT 0.5 mM PMSF 0.2 mM EDTA
具体操作流程请点击下面的链接下载：
Gel Mobility Shift Analysis .pdf
