One of the possible applications of human induced pluripotent stem (iPS) cells is its usage for transplantation (Takahashi K et al. Cell 131:861–872, 2007; Yu J et al. Science 318:1917–1920, 2007; Park IH et al. Nature 451:141–146, 2008; Lowry WE et al. Proc Natl Acad Sci USA 105:2883–2888, 2008; Morita S et al. Gene Ther 7:1063–1066, 2000). For this purpose, animal components and undefined factors should be precluded. In this chapter, we describe a practical and effective protocol for generating and maintaining human iPS cells on autologous feeders.
