With the development of the Internet, a growing number of two-dimensional electrophoresis (2DE) databases have become available (60 in 2009, for a total of 425 image maps). By linking the two components constituting 2DE databases, gel images and protein information, the active hypertext links provide a powerful tool for data integration, in addition to navigation from one database to another. This chapter shows how to prepare the necessary files to build a federated 2DE database in order to make it available over the Internet and how to further update it.
