| Cloning by Limiting Dilution of Hybridoma |
| Author:Nanci Donacki |
| Source:Contributed by Nanci Donacki |
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| Materials DMEM, high glucose (Life Technologies, Inc. #10313-021 or equivalent) Fetal Bovine Serum (Life Technologies, Inc. #16000-044 or equivalent) L-glutamine (Life Technologies, Inc. #25030-149 or equivalent) Hybridoma Cloning Factor (Fisher # IG50-0615) 50 ml sterile centrifuge tubes ( Falcon #2070) 15 ml sterile centrifuge tubes (Falcon # 2099) 96 well culture plates (Falcon #3072)) Hemocytometer Trypan Blue, 0.4% (Life Technologies, Inc. # 630-5150AG) Multi-channel pipettor and sterile tips Reagent Reservoir HT (Life Technologies, Inc. #11067-030) |
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