CHO-K1 (hamster, Chinese, ovary)
ECACC 85051005
Morphology: Epithelial
Hamster Chinese ovary
Depositor: Dr D Newell, PHLS CAMR, Porton Down, Salisbury, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Nutritional and gene expression studies
Available in the following LABORATORY:
CAMR Centre for Applied Microbiology & Research (ECACC, Salisbury, Wiltshire)
Ham's F12 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:4 to 1:10 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C. Passages: 17 Karyotype: Hypodiploid, modal No 20
Hazard: CY
A subclone of the parental CHO cell line, which was derived from the ovary of an adult Chinese hamster. Cells require proline due to the absence of the gene for proline synthesis, the block in the biosynthetic chain lies in the step converting glutamic acid to glutamine gamma serialdehyde. They undergo morphological changes in response to cholera toxin.
Further information:
Research council deposit: No
Price_code: A
Availability in cell line catalogues: ATCC CCL 61; IZSBS BS CL15; DSMZ ACC 110;
Bibliographic references:
J Exp Med 1958;108:945 Proc Nat Acad Sci USA 1968;60:1275
CHO-K1 (hamster, Chinese, ovary)
Morphology: epithelial-like
Species: hamster, Chinese; Tissue: ovary; Original line: CHO
Properties: genetics; nutrition; transfection; toxicity screening; cell biology; virology; cytotoxicity; bacterial cytotoxicity; gene expression; Susceptible to: arbovirus, vesic. stomatitis (Indiana), Getah arbovirus, mengovirus
Available in the following LABORATORY:
Russian Academy of Medical Sciences (MWIIW, Moscow)
monoclonal continuous culture, grown as monolayer
Further information:
Karyology: hypodiploid, modal number 20
Availability in cell line catalogues: ATCC CCL 61; ECACC 85051005; IZSBS BS CL15; DSMZ ACC 110;
Bibliographic references:
J Exp Med 1958;108:945
Proc Natl Acad Sci U S A 1965;52:288 - PMID: 5244736
Proc Natl Acad Sci U S A 1968;60:1275 - PMID: 5244736
Virology 1965;27:11 - PMID: 4283990
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