Sample preparationWash embryos 1 x in 25% MMRRemove excess bufferAdd 10 volumes &q...
生物知识
生物知识
生物知识
Applications of Chemical Tagging Approaches in Combination with 2DE and Mass Spectrometry
Chemical modification reactions play an important role in various protocols for ma...
生物知识
核纤层蛋白(nuclear lamina protein)
脊椎动物细胞中有三种类型的核纤层蛋白(A,B,C), 核纤层蛋白A和C是由同一个转录单位编码的, 只不过是通过可变剪接形成不同的mRNA。它们在肽链上的差别是:核纤...
生物知识
多能干细胞（pluripotent stem cell）
多能干细胞具有分化出多种细胞组织的潜能，但失去了发育成完整个体的能力，发育潜能受到一定的限制。骨髓多能造血干细胞是典型的例子，它可分化出至少十二种血细胞，但不能分化...
生物知识
Assaying Cell-Cell Adhesion
A major feature inDictyosteliumdevelopment is the transition from the unicellular ...
生物知识
Quantitation of Cocaine, Benzoylecgonine, Ecgonine Methyl Ester, and Cocaethylene in Urine and Blood
Cocaine, a stimulant, is a commonly abused drug. Cocaine and its metabolites are m...
生物知识
Genetic Modification of Murine Hematopoietic Stem Cells by Retroviruses
Among the currently available methods for gene transfer, recombinant murine retrov...
生物知识
Protocols for Use of Homologous Recombination Gene Targeting to Produce MicroRNA Mutants in Drosophi
MicroRNAs (miRNAs) are noncoding RNA molecules that have come to attract considera...
生物知识
Gel Electrophoresis Assays for Analyzing DNA Double-Strand Breaks in Saccharomyces cerevisiae at Var
Meiotic recombination is triggered by programmed DNA double-strand breaks (DSBs), ...
生物知识
Microautophagy in the Yeast Saccharomyces cerevisiae
Microautophagy involves direct invagination and fission of the vacuolar/lysosomal ...
