生物知识 Novel Strategies for the Identification of Clock Genes Neurospora With Insertional Mutagenesis As the molecular mechanism of circadian clocks has reached high complexity, the fu... 05月03日 发表评论 阅读全文
生物知识 Approaches and Species in the History of Vertebrate Embryology Recent debates about model organisms echo far into the past; taking a longer view ... 05月03日 发表评论 阅读全文
生物知识 Semipermeabilized Cells to Study Procollagen Assembly This chapter will describe the preparation and use of a semipermeabilized (SP) cel... 05月03日 发表评论 阅读全文
生物知识 Joining RNA Molecules with T4 DNA Ligase Researchers interested in studying RNA structure and function or RNA-protein inter... 05月03日 发表评论 阅读全文
生物知识 Methods to Study the Ras2 Protein Activation State and the Subcellular Localization of Ras-GTP in Sa Ras proteins were highly conserved during evolution. They function as a point of c... 05月03日 发表评论 阅读全文
生物知识 协助扩散 物质通过质膜进出细胞的方式之一。物质必须在载体蛋白的协助下,才能从高浓度一侧通过质膜向低浓度一侧扩散,但此种方式不消耗代谢能。协助扩散和自由扩散都是物质顺浓度梯度的... 05月03日 发表评论 阅读全文
生物知识 Isolation and Characterization of Anthocyanin 5-O-Glucosyltransferase in Perilla frutescens var. cri The glucosylation of 5-position of anthocyanin molecules is one of most important ... 05月03日 发表评论 阅读全文
生物知识 A Detailed Protocol for Chromatin Immunoprecipitation in the Yeast Saccharomyces cerevisiae Critical cellular processes such as DNA replication, DNA damage repair, and transc... 05月03日 发表评论 阅读全文
生物知识 Use of Liposomes to Evaluate the Role of Membrane Interactions on Antioxidant Activity Cellular membranes, which contain abundant phospholipids, such as phosphatidylchol... 05月03日 发表评论 阅读全文
生物知识 Screening for Protein-Protein Interactions in the Yeast Two-Hybrid System in Embryonic Stem Cells The two-hybrid system (THS) (1 ) is a molecular genetic screen that detects protei... 05月03日 发表评论 阅读全文