生物知识 Convergent Extension Analysis in Mouse Whole Embryo Culture Mutations have been identified in a non-canonical Wnt signalling cascade (the plan... 05月05日 发表评论 阅读全文
生物知识 Methods for Detection of ProteinProteinnl and ProteinDNA Interactions Using HaloTag HaloTag™ is a protein fusion tag which was genetically engineered to covale... 05月05日 发表评论 阅读全文
生物知识 Measuring Transport and Accumulation of Radiolabeled Substrates in Oocytes and Embryos Radiolabeled compounds that are substrates for transmembrane transporters can be u... 05月05日 发表评论 阅读全文
生物知识 Endothelial and Cardiac Regeneration from Adipose Tissues For a long time, adipose tissue was only considered for its crucial role in energy... 05月05日 发表评论 阅读全文
生物知识 Generation and Detection of S-Nitrosothiols Nitric oxide (NO) plays a pivotal role in cellular signaling in many different org... 05月05日 发表评论 阅读全文
生物知识 Production and Characterization of Immortal Human Neural Stem Cell Line with Multipotent Differentia We document the protocols and methods for the production of immortalized cell line... 05月05日 发表评论 阅读全文
生物知识 EGF受体(EGF receptor) EGF受体是一种糖蛋白, 广泛分布于哺乳动物的上皮细胞、人的成纤维细胞、胶质细胞、角质细胞等。EGF 受体是一条含有1186个氨基酸残基的多肽链, 相对分子质量为1... 05月05日 发表评论 阅读全文
生物知识 全血细胞培养染色体技术常用试剂配制 一、国内实验室应用试剂配制 (一)培养液 (1)RPMI1640培养液:称取RPMI培养基9.8g,碳酸氢钠1.9g、青霉素100u/ml,加双蒸水至1000ml,... 05月05日 发表评论 阅读全文
生物知识 In Vivo Evaluation of Putative Hematopoietic Stem Cells Derived from Human Pluripotent Stem Cells Efficient derivation and isolation of hematopoietic stem cells (HSCs) from human p... 05月05日 发表评论 阅读全文
生物知识 Retroviral Transduction of Murine Hematopoietic Stem Cells Hematopoietic stem cells (HSC) are inherently rare cell types that cannot be obtai... 05月05日 发表评论 阅读全文