生物知识 Expression of Mammalian Membrane Proteins in Mammalian Cells Using Semliki Forest Virus Vectors One of the major bottlenecks in drug screening and structural biology on membrane ... 05月05日 发表评论 阅读全文
生物知识 Primary Culture of Human Antral Endocrine Cells The mucosal endocrine cells in the antrum are found as individual elements intersp... 05月05日 发表评论 阅读全文
生物知识 Quantitative Analysis of Protein-RNA Interactions by Gel Mobility Shift The gel mobility shift assay is routinely used to visualize protein—RNA interactio... 05月05日 发表评论 阅读全文
生物知识 Three-Dimensional Interphase Analysis Enabled by Suspension FISH An established method for performing the entire FISH procedure in suspension inste... 05月05日 发表评论 阅读全文
生物知识 Enrichment of Cells in Different Phases of the Cell Cycle by Centrifugal Elutriation Understanding the molecular and biochemical basis of cellular functions involved i... 05月05日 发表评论 阅读全文
生物知识 Monolithic Bioreactors for Macromolecules Enzymes immobilized on solid-phase matrices have found various applications in bio... 05月05日 发表评论 阅读全文
生物知识 Detection and Quantitation of mRNAs Using Ribonuclease Protection Assays The ribonuclease protection assay (RPA) is a sensitive and straightforward method ... 05月05日 发表评论 阅读全文
生物知识 Mass Production of Drosophila Embryos and Chromatographic Purification of Native Protein Complexes The purification of native protein complexes requires the availability of sufficie... 05月05日 发表评论 阅读全文
生物知识 Experimental Models to Analyze Differentiation Functions of Cultured Keratinocytes In Vitro and In V In this chapter, we present technical details for the generation of in vitro skin ... 05月05日 发表评论 阅读全文
生物知识 Analysis of the Fission Yeast Schizosaccharomyces pombe Cell Cycle Fission yeast is a popular model organism for the study of the cell cycle. It grow... 05月05日 发表评论 阅读全文