Human embryonic stem cells (hESCs) are pluripotent cells derived from the embryo a...
生物知识
生物知识
生物知识
Methods for Homologous Recombination in Drosophila
We present detailed protocols for two methods of gene targeting inDrosophila. The ...
生物知识
Introduction: Why Analyze Single Cells
Powerful methods in molecular biology are abundant; however, in many fields includ...
生物知识
In Vitro and Ex Vivo Gene Delivery to Cells by Electroporation
Electroporation generally refers to the technique of permeabilizing cell membranes...
生物知识
Detection of c-myc Amplification in Formalin-Fixed Paraffin-Embedded Tumor Tissue by Chromogenic In
In situ hybridization (ISH) allows evaluation of genetic abnormalities, such as ch...
生物知识
Polyamine Transport Systems in Mammalian Cells and Tissues
Polyamine transport plays an important role in the homeostatic regulation of the p...
生物知识
DNA Fragmentation Assays for Apoptosis
Protocol I:Triton X-100 Lysis BufferIn 96 flat-wells plate, incubate 4x10 6 target...
生物知识
细胞学技术的一般环节
一、纺锤体阻断剂（Spindle inhibitor） 在有丝分裂过程中，随着纺锤丝的形成，染色体被牵引到一起难以观察其形态。纺锤体的形成在于细胞质和纺锤体成分的粘...
生物知识
Lanthanide-Based Upconversion Nanoparticles for Connexin-Targeted Imaging in Co-cultures
From the perspective of deep tissue imaging, it is required that the excitation li...
生物知识
Digital Imaging of Stem Cells by Electron Microscopy
This chapter deals with basic techniques of scanning and transmission electron mic...
