简介小鼠处死前大量采血可用腹主动脉采血法。使用腹主动脉采血法一次可采血达 1 mL，血样污染少，缺点是操作要求较高，需专业人员统一操作。材料与仪器器材：① 解剖板②...
生物知识
生物知识
生物知识
消化系统组织学分析实验-切片观察实验
简介在解剖学观察的基础上，可以通过对肉眼可见的病变部位进行切片染色来判断具体的病理缺陷，如癌症的浸润程度，炎症的发展程度等，也可以通过病理学评判标准对其程度进行统计...
生物知识
消化系统组织学分析实验-免疫组化（荧光）确定上皮增生及细胞浸润
简介对于癌症病变的组织学分析，在早期可以通过5-溴脱氧尿嘧啶核苷（BrdU）掺入测定目的细胞的增殖能力，如胃上皮增殖及肠道干细胞向上增殖能力，推测其转化的可能性。由...
生物知识
Tracking Gene-Modified T Cells In Vivo
Identification, monitoring, and analysis of genetically modified cells in the peri...
生物知识
Confocal Imaging Protocols for Live/Dead Staining in Three-Dimensional Carriers
In tissue engineering, a variety of methods are commonly used to evaluate survival...
生物知识
Purification of a Membrane Protein (Ca2+/Mg2+-ATPase) and Its Reconstitution into Lipid Vesicles
Purification of membrane proteins of necessity requires the use of detergents to s...
生物知识
Wound healing assay
The wound healing assay allows the researcher to study cell migration and cell int...
生物知识
Quantification of Xenoprotein Electroinsertion in Mammalian Cells
Recent studies reported the so-called “electroinsertion” of proteins having a memb...
生物知识
Luminescent Biosensors for Real-Time Monitoring of Intracellular cAMP
G-protein coupled, seven-transmembrane (7-TM) receptors (GPCRs) comprise a diverse...
生物知识
Clonogenic Assays for Hematopoietic and Tumor Cells Using Agar-Containing Capillaries
The so-called hematopoietic stem cells from mouse and human bone marrow can be ind...
