1. 实验进行前，无菌室及无菌操作台(laminar flow) 以紫外灯照射30-60 分钟灭菌，以70 % ethanol 擦拭无菌操作抬面，并开启无菌操作台风...
生物知识
生物知识
生物知识
Preparation of cytoplasmic extracts for the application in a cell-free system
Description Cells are grown to 80% confluency, then harvested, washed and disrupte...
生物知识
Techniques in Embryoid Body Formation from Human Pluripotent Stem Cells
Embryoid bodies (EBs) can be generated by culturing human pluripotent stem cells i...
生物知识
染色体早熟凝集(premature chromosome condensation,PCC)
将处于分裂期(M期)的细胞与处于细胞周期其他阶段的细胞融合, 使其他期细胞的染色质提早包装成染色体, 这种现象称为染色体早熟凝集(premature chromos...
生物知识
Determination of Polyamines by Capillary Electrophoresis
The polyamines, mainly putrescine (PU), spermidine (SPD), and spermine (SPM) (Fig....
生物知识
Analysis of Histone Posttranslational Modifications from Nucleolus-Associated Chromatin by Mass Spec
Chromatin is unevenly distributed within the eukaryote nucleus and it contributes ...
生物知识
Plasma Nitrite and Nitrate Determination
The concentrations of nitrite and nitrate in blood plasma are important in clinica...
生物知识
Growth of Human Embryonic Stem Cells in Long-Term Hypoxia
Human embryonic stem cells (hESCs) hold a great promise for regenerative medicine ...
生物知识
Directed Differentiation of Human Embryonic Stem Cells to Dendritic Cells
Embryonic stem cells represent a pluripotent population of cells capable of self-r...
生物知识
Cloning and Expression Analyses of Pig Genes
Understanding the molecular bases of xenograft rejection is one of the highest pri...
