关于离子交换层析实验的介绍，对于常用到的层析介质的一些基本技术数据罗列如下：离子交换介质名称最高载量颗粒大小（μm ）特性/ 应用pH 稳定性工作耐压（MPa ）最...
生物知识
生物知识
生物知识
Purification of Calmodulin from Bovine Brain
References:Watterson et al.(1980)J Biol Chem 255:962-75.Charbonneau et al.(1979)Bi...
生物知识
Protein Expression and Purification of MBP-T antigen (131-259)
Comments: T antigen (131-259)is an MBP-Hisx6-fusion protein constructed by Laura M...
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Yeast protein prep for SDS PAGE and western (rapid)
Horvath and Riezman,Yeast,1994; Gottschling LabSample Buffer:10 ml0.06M Tris-HCl,p...
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Purification of GST fusion proteins in E.coli GST融合蛋白纯化，方法二
GST Protein Prep.Ver.21)Grow 50ml of culture in LB or TB antibiotic o/n at 37℃ sha...
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Yeast Nuclear Extract (Small Scale)
Yeast Nuclear Extract (Small Scale)Hahn Lab (adapted from J.Leatherwood,Ptashne la...
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TCA protein precipitation
Kyle Coachman/Linda Warfield/Steve HahnLast modified 5/13/02To concentrate protein...
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Urea Lysis Protocol
9M Urea,2.5mM EDTA,2.5mM EGTA,1% DTE,4% CHAPSmake 10ml and aliquot 10x1ml,freeze a...
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Preparation of protein extracts for western blot
1.Grow cells to mid-log (OD600 less or equal to 1.0).2.Harvest about 5 OD's o...
生物知识
Purifying Protein from Inclusion Bodies
Buffer ABuffer B50 mM tris-HCl, pH 8.020 mM Na2 HPO4 , pH 7.25 mM EDTA20 mM NaCl10...
