Section of Cancer Genomics,Genetics Branch,NCINational Institutes of HealthReagent...
生物知识
生物知识
生物知识
RNase Protection Assay (Bowtell Lab)
1. Cut 10ug of plasmid DNA where you want the transcript to end.2. Add an equal vo...
生物知识
煮沸裂解法小量制备质粒DNA
经Triton X-100、溶菌酶和加热处理可从小量（1～2ml）细菌培养物中分离质粒DNA所获得的质粒则可以用电泳或限制性内功核酸酶消化的方法鉴定；经PEG处理进...
生物知识
Phenol/chloroform Extraction of DNA
Materials:phenol:chloroform (1:1)chloroformAdd an equal volume of buffer-saturated...
生物知识
Use of Internal and External Standards or Reference RNAs for
IntroductionNorthern blotting, nuclease protection assays and RT-PCR are frequentl...
生物知识
Isolation of Retroelement from Plant Genomic DNA (PCR法从植物寻找逆转录元件)
Retroelements and their derivatives are a ubiquitous and abundant component of pla...
生物知识
Plasmid DNA Isolation, Alkaline Lysis（碱裂解法分离质粒DNA）
Reagents:Soln 1: 50 mM glucose/10 mM EDTA/25 mM Tris pH 8. Autoclave before use. A...
生物知识
Extraction of Genomic DNA from Mouse Tail
1、Put a 0.5-1.5 CM MOUSE TAIL SNIP in a 1.5 ml eppindorf tube and store frozen unt...
生物知识
A quick method to isolate plant
Use from 0.01 - 0.1 gram plant material.Grind the plant material with liq.N2 in a ...
生物知识
植物基因组DNA提取
【实验目的】 掌握植物总DNA的抽提方法和基本原理。学习根据不同的植物和实验要求设计和改良植物总DNA抽提方法。 【实验原理】 通常采用机械研磨的方法破碎植物的组织...
