Carbonate Solution For Western Blot

CARBONATE WESTERN TRANSFER SOLUTIONSDunn, Anal. Biochem. 1986: 157

1.5L 1.0L
10mM NaHCO3 1.26g chill to 4oC 0.84g
3mM Na2CO3 0.477g pH ~9.9 0.318g
in 20% MeOH 300mL 200mL

1. Blot: no need to presoak gell.Transblot: 1-2H @ 1A keep T<35oCMiniblot: 1H @ 250mA 2. Assemble wet sandwich on black side:black side holderbrillo pad3MM papergelnitrocellulose3MMbrillowhite side holder

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