Dictyostelium discoideumis one of the most facile eukaryotic systems for the study of chemotactic response to secreted chemical ligands.Dictyosteliumgrow as individual cells, using bacteria and fungi as primary nutrient sources; during growth,Dictyosteliummoves directionally toward folate, a bacterial byproduct. Upon nutrient depletionDictyosteliuminitiates a multicellular development program characterized by the production and secretion of cAMP. Cell surface receptors specifically recognize extracellular cAMP, which serves as both a morphogen to promote development and a chemoattractant to organize multicellularity. We discuss several approaches for the study of ligand–receptor interaction, with focus on affinity class determination and quantification of ligand binding sites (i.e., receptors) per cell. We further present examples for the application of biochemical assays to characterize the ligand-induced kinase activation of PI3K, GSK3, and ERK2.
