β-羟丁酸试剂盒可检测各种生物样品内的β-羟丁酸含量，快速、灵敏、准确！你的首选！
丙酮、乙酰乙酸和β-羟丁酸（β-Hydroxybutyrate， β-HB）统称为酮体（ketone body），当胰岛素水平下降到非常低的水平时，发生糖尿病酮症酸中毒，关闭葡萄糖供给身体。生理反应是由脂肪酸氧化产生的乙酰辅酶A产生酮体（主要是β-羟基丁酸酯，占75%以上）。酮体生产率非常高，超过了身体利用它们作为能量来源的能力，血液浓度越来越高，称酮血症（ketonemia）。过多的酮体从尿中排出，称酮尿（Ketonuria）。因酮体中除丙酮外，均为强酸物质，过多的酮体由尿排出时，必须与体内碱储（主要是Na+）结合成盐类，因此体内碱储消耗过多，最后导致代谢性酸中毒及脱水，这种病理变化称为酮症（Ketosis）。
如何检测样品中β-羟丁酸的含量的试剂盒呢？
艾美捷科技为您推荐享誉全球市场的β-羟丁酸（β-HB）定量分析试剂盒： K632-100
原理：β-羟丁酸（β-HB）检测试剂盒利用β-羟丁酸脱氢酶参与反应，将样本内的β-羟丁酸催化脱氢，其产物能与发光探针反应产生450nm处的吸收峰，吸光值与其含量成正比，本试剂盒可检测各种生物样品。
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| 产品名称及描述 | 货号 | 产品说明 |
| β-Hydroxybutyrate (β-HB) Colorimetric Assay Kit | K632-100 | 详情 |
*β-羟丁酸试剂盒启用后，按照要求条件保存各个组分，并在2个月内用完。
β-羟丁酸试剂盒特点：
- 步骤简单，只需40分钟
- 检测范围：01-0.2 mM
- 样品类型：血清，血浆，细胞裂解液，尿液
Tips：
- 推荐使用肝素（Heparin）收集血液
- 对于样品可能存在的检测干扰物，建议使用10kDa的超滤管，过滤，收集澄清的样品
- 血清样品可直接检测，其它样品需要做梯度稀释，确保检测结果在标准曲线范围内
《卓越的标准曲线》
引用文献
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Garg, Manisha et al. (2017) Liraglutide acutely suppresses glucagon, lipolysis and ketogenesis in type 1 diabetes, Diabetes Obes Metab. 2017 Mar 17.
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Huijsman E et al (2009) Am J Physiol Endocrinol Metab; 10.1152/ajpendo.00190.2009.
Janna A. van Diepen et al., Aspirin reduces hypertriglyceridemia by lowering VLDL-triglyceride production in mice fed a high-fat diet, Am J Physiol Endocrinol Metab, Dec 2011; 301: E1099 - E1107.
Ji et al., Molecular and metabolic profiles suggest that increased lipid catabolism in adipose tissue contributes to leanness in domestic chickens.Physiol Genomics, May 2014; 46: 315 - 327.
Leigh Goedeke et al., A Regulatory Role for MicroRNA 33* in Controlling Lipid Metabolism Gene Expression.Mol. Cell. Biol., Jun 2013; 33: 2339 - 2352.
Park, S. et. al. A ketogenic diet impairs energy and glucose homeostasis by the attenuation of hypothalamic leptin signaling and hepatic insulin signaling in a rat model of non-obese type 2 diabetes. Exp Biol Med, Feb 2011; 236: 194 - 204.
Takimoto & Hamada. Acute exercise increases brain region-specific expression of MCT1, MCT2, MCT4, GLUT1, and COX IV proteins. J Appl Physiol, May 2014; 116: 1238 - 1250.
Wu, S. et. al. Insulin Resistance Secondary to a High-Fat Diet Stimulates Longitudinal Bone Growth and Growth Plate Chondrogenesis in Mice. Endocrinology, Feb 2011; 152: 468 - 475.
Wu. S. et. al. Insulin Resistance Secondary to a High-Fat Diet Stimulates Longitudinal Bone Growth and Growth Plate Chondrogenesis in Mice. Endocrinology, Nov 2010; 10.1210/en.2010-0803.
艾美捷科技与国内外优秀的生物试剂供应商保持着密切的合作关系，目前已成为众多国际知名品牌的中国总代理或一级代理，主要包括：Abbkine、Merck Millipore、Origene、AAT Bioquest、Cayman Chemical、Abnova 、Biovision、ProSpec、Hycult Biotech 、Equitech-Bio、Trevigen、Alomone Labs、Epigentek、ImmunoReagents 、Jackson、SouthernBiotech、US Biological 、Caisson Labs等，可以在第一时间为用户提供最前沿的专业资讯、完备的产品及物流服务。
