Construction and Use of Compartmented Cultures for Studies of Cell Biology of Neurons

The compartmented culture method has numerous features that make it useful for studies of nerve fiber growth, among which are:

1. Distal axons can be exposed to a different fluid and cellular environment than cell bodies and proximal axons, which is useful for studies of trophic, ionic, and pharmacological regulation of growth.
2. Distal axons can be removed (neuritotomy), and subsequently regenerate, which permits many useful approaches to the study of axon growth and regeneration.
3. Axons can be chronically electrically stimulated during growth and regeneration.

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